clpP [2020-06-11 08:55:50]

ATP-dependent Clp protease proteolytic subunit (class III heat-shock protein)

locus: BSU_34540

pI: 5.00

mw: 21.00 kDa

protein length: 197 aa

gene length: 594 bp

function: protein degradation

product: ATP-dependent Clp protease proteolytic subunit

essential: no

ec: 3.4.21.92

synonyms: yvdN

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.7|Proteolysis] → [category|SW 3.3.7.4|Additional proteins involved in proteolysis]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.4|Heat shock proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]

Gene

Coordinates: 3,546,234 → 3,546,827

Phenotypes of a mutant

• increased thermotolerance due to increased stabiliy of [protein|2C6386E9A63F410558D168798D077DF91590F454|Spx] and thus increased expression of ''[gene|4E5C84FDC8FE2FEFF47306C91ECAB7F17D3E38E9|trxA]'' [Pubmed|24417481]
• the mutation suppresses the heat sensitivity of spores overexpressing ''[gene|85DE3CB6CDA61C141C6030367C0A13BB642160B9|cmpA]'' [Pubmed|26387458]
• non-motile [Pubmed|27014237]

The protein

Catalyzed reaction/ biological activity

• Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec, and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs) (according to UniProt)

Protein family

• Peptidase S14 family (with [protein|A16C6515D185E3A268D57D77F28EF7EF9AF9FE05|TepA], according to UniProt)

Modification

• phosphorylated on Arg-13 [Pubmed|22517742]

Effectors of protein activity

• the novel antibiotic ADEP (acyldepsipeptides) dysregulates [protein|CB06A70DE7462CEB7AF5D8C28943C878DD56DE1A|ClpP] activity and allows [protein|41872E2EF00C79918DD077F2EF78F37E24FEB110|FtsZ] degradation in the absence of an ATPase subunit ([protein|86A2F2F65290F4471D6FD03B694821C66C180D8A|ClpC], [protein|8C5B14FE5E03427F9A598C75D4081FA0D6696299|ClpE], or [protein|297F53DAD3351E0C55108DD2C93B78FFB174438C|ClpX]) [Pubmed|21969594]

Structure

• [PDB|3KTG] [Pubmed|20305655]

[SW|Localization]

• cytoplasmic polar clusters, excluded from the nucleoid, induced clustering upon heat shock, colocalization with [protein|297F53DAD3351E0C55108DD2C93B78FFB174438C|ClpX], [protein|86A2F2F65290F4471D6FD03B694821C66C180D8A|ClpC] and [protein|8C5B14FE5E03427F9A598C75D4081FA0D6696299|ClpE] [Pubmed|18786145,18689473]

additional information

• belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

Expression and Regulation

Operons

• genes: [gene|CB06A70DE7462CEB7AF5D8C28943C878DD56DE1A|clpP]
  description: [Pubmed|9643546]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|9643546], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|9643546,11544224], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]

  regulatory mechanism

  • [protein|908DB17A39D518E84977250C55825E77FA02E391|CtsR]: repression, [Pubmed|9987115,11179229,16163393,17380125], in [regulon|908DB17A39D518E84977250C55825E77FA02E391|CtsR regulon]

  regulation

  • induced by heat ([protein|search|CtsR]) [Pubmed|9987115]

Biological materials

Mutant

• ''clpP::spec'' and ''clpP::cat'', available in the [SW|Leendert Hamoen] lab
• BP99 (''clpP''::''tet''), available in [SW|Fabian Commichau]'s lab [Pubmed|25610436]
• GP551 (spc), available in [SW|Jörg Stülke]'s lab
• QB4916 (spc), available in [SW|Ulf Gerths]'s and [SW|Jörg Stülke]'s labs
• 1S139 (''clpP''::''erm''), available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S139&Search=1S139 BGSC]
• 1S140 ( ''clpP''::''spec''), available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S140&Search=1S140 BGSC]
• BKE34540 (''[gene|CB06A70DE7462CEB7AF5D8C28943C878DD56DE1A|clpP]''::''erm'', available in the BGSC and in [SW|Jörg Stülke]'s lab) [pubmed|28189581]
• GP1822 and GP1786 (''[gene|CB06A70DE7462CEB7AF5D8C28943C878DD56DE1A|clpP]''::''erm'', available in [SW|Jörg Stülke]'s lab)
• GPUG1 (erm), available in [SW|Ulf Gerth]'s and [SW|Jörg Stülke]'s labs
• BKE34540 (Δ[gene|CB06A70DE7462CEB7AF5D8C28943C878DD56DE1A|clpP]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE34540 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATGCTCCTCCTTCACC, downstream forward: _UP4_TAATAACACAACCTGCAAGA
• BKK34540 (Δ[gene|CB06A70DE7462CEB7AF5D8C28943C878DD56DE1A|clpP]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK34540 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATGCTCCTCCTTCACC, downstream forward: _UP4_TAATAACACAACCTGCAAGA

GFP fusion

• C-terminal GFP fusions (both single copy and as 2th copy in ''amyE'' locus, also as CFP and YFP variants) available in the [SW|Leendert Hamoen] lab

Antibody

• available in [SW|Ulf Gerth]'s and [SW|Jörg Stülke]'s labs

labs

• [SW|Leendert Hamoen], Newcastle University, UK [http://www.ncl.ac.uk/camb/staff/profile/l.hamoen homepage]

References

Reviews

• 16211032,17302811,23375660,23479438,19609260,19781636,26639779,28748186

Original Publications

• 9643546,10809708,11807061,9987115,14679237,18689476,15317791,17586624,11684022,12923101,17560370,16899079,19226326,20070525,9987115,11544224,14763982,9643546,19767395,11112444,9535081,18689473,20305655,20852588,16200071,21969594,22080375,22517742,17380125,12598648,9890793,20049702,20049702,23927726,24263382,24226776,24417481,15378759,23361916,24942655,25212124,25433860,25610436,26387458,27014237,27669037,28760849,29186773,29625553,28333276,31362989,32181548,32442436